Molecular mechanism of MLF1 in AC16 cell line

Previous studies found that MLF1 mRNA levels tended to decline in aging hearts. Functional studies found that knockdown of MLF1 ameliorated the drug-induced cellular senescence phenotype. Here, we apply transcriptomics to explore the underlying molecular mechanisms. Overall design: Cardiomyocyte AC16 cells were cultured in DMEM/F12 and DMEM (high glucose), respectively. DMEM supplemented with 10% fetal bovine serum (FBS, Gibco, USA), 100 U/mL penicillin, and 100 µg/ml streptomycin. Medium without FBS was replaced before transfection. Negative control siRNA and Mlf1 siRNA were transfected into AC16 cells at ~70% confluence using jetPRIME® (Polyplus-transfection, NY, USA). After 12 h of transfection, fresh complete medium was replaced. RNA extracted according to the manufacturer's instructions using Trizol reagent. RNA libraries were prepared for sequencing according to manufacturer's instructions of Beijing Genomics Institution (BGI)