Mapping the Melanoma Plasma Proteome (MPP) using Single-Shot Proteomics and Custom Protein Databases

We mapped the Melanoma Plasma Proteome (MPP) of undepleted samples a new strategy for protein identification refered here as Wise MS transfer (WiMT). We were able to identify more than 1,000 protein in undepleted plasma samples which shows a deep coverage of the plasma proteome. The MPP of undeplted samples was well characterized showing a great potential for large-scale screening of melanoma proteomics studies and other neoplastic diseases.