H3K27Ac ChIP-seq profile of human oral epithelial cell line HIOEC

To identify the active enhancers and promoters in human oral epithelial cell line, we performed H3K27Ac ChIP-seq for HIOEC cell line. HIOEC cells were maintained in culture medium supplemented with 1.2mM Ca2+ for 3 days, and then were disassociated with 0.25% trypsin-EDTA. For each replicates, 500000 HIOEC cells were harvested for H3K27Ac ChIP-seq library preparation. Three independent biological replicates were performed.