Gene expression profile at single nuclei level of cells from mouse hippocampus

We performed single-nucleus RNA-seq and chromatin accessibility analyses (snRNA-seq and ATAC-seq) in the hippocampus of wild-type (WT) mice and mice carrying amyloid precursor protein knock-in mutations (APPNL-G-F) in response to chronic wheel-running exercise. 8-week old female WT and APPNL-G-F mice were put into cages with running wheel for voluntary exercise for 10 weeks. Mice from 4 different conditions (WT_RT, WT_EX, APP_RT, APP_EX) were subjected to single nuclei RNA and ATAC sequencing.我们针对慢性转轮运动干预，在野生型（wild-type，WT）小鼠与携带淀粉样前体蛋白敲入突变（APPNL-G-F）的小鼠的海马组织中，开展了单细胞核RNA测序（single-nucleus RNA-seq，snRNA-seq）与染色质开放程度分析（ATAC-seq）。将8周龄的雌性WT及APPNL-G-F小鼠置于配备转轮的笼具中进行自主运动，持续时长为10周。最终对来自4组不同实验条件（WT_RT、WT_EX、APP_RT、APP_EX）的小鼠样本开展单细胞核RNA测序与ATAC测序。