Spatial compartmentalization of signalling imparts source-specific functions on secreted factors.

Efficient regeneration requires multiple cell types acting in a coordination. To better understand the intercellular networks involved and how they change when regeneration fails, we profiled the transcriptome of hematopoietic, stromal, myogenic, and endothelial cells over 14 days following acute muscle damage. Hematopoietic, stromal, myogenic, and endothelial cells were collected over 14 days following acute muscle damage and subjected to RNAseq. We used a novel bioinformatics pipeline to generate a time-resolved computational model of interactions.