NGS- IL-12 treated primary murine neuronal cultures

We performed next generation sequencing of primary murine neurons stimulated with recombinant IL-12 to test the isolated effect of IL-12 on neurons, independent from a heavily inflamed CNS microenvironment. Principal component analysis (PCA) revealed a dose-dependent response to IL-12 in the neuronal transcriptome. Our computational analysis identified 1,878 significantly DEGs (p≤0.01, log ratio ≥0.5). Several of the upregulated genes have been linked to promoting neurogenesis (Fpr1, Mbdi1), neuroprotection (Timp1, Angptl4) and enhancing remyelination (Timp1, Matn2), thereby suggesting a direct neuroprotective and neurotrophic effect of IL-12.

Sequencing raw data and processed gene expression data will be deposited into the GEO repository upon publication. Code for analyses will be available upon contacting the corresponding authors: [e-mail] and [e-mail]

Sequencing raw data and processed gene expression data will be deposited into the GEO repository upon publication.

本研究对经重组IL-12刺激的原代小鼠神经元进行了下一代测序，旨在检验IL-12对神经元的影响，排除中枢神经系统炎症微环境的影响。主成分分析（PCA）揭示了神经元转录组对IL-12的剂量依赖性反应。我们的计算分析鉴定出1,878个差异表达基因（DEGs），其p值≤0.01，对数比≥0.5。其中，部分上调基因与促进神经发生（Fpr1、Mbdi1）、神经保护（Timp1、Angptl4）和增强髓鞘再生（Timp1、Matn2）相关联，从而提示IL-12具有直接的神经保护和神经营养作用。测序原始数据和经过处理的基因表达数据将在发表后存入GEO数据库。分析代码可通过联系相应作者获得：[e-mail] 和 [e-mail]。测序原始数据和经过处理的基因表达数据将在发表后存入GEO数据库。