Time Course Crude Oil Exposures of Early Life Stage Mahi-Mahi for RNA-Seq and Assessment of Cardiotoxicity

Three 96 h time course exposures were performed on freshly fertilized mahi-mahi embryos using two types of crude oil (slick (OFS) and source (Mass)) for the purposes of: (1) analyzing whole animal transcriptome responses by RNA-Seq/qPCR analysis, (2) phenotypic anchoring of transcriptional responses to cardiotoxic endpoints by imaging analysis of heart rate and pericardial and yolk sac edema and (3) obtaining additional replicates for qPCR to confirm transcriptional response of select genes identified by RNA-Seq. This dataset contains all of the data associated with the exposures and phenotypic analyses. This dataset also includes level thresholds (e.g., LC25, LC50) for two different weathered states of oil. Tests G001 and G002 were performed with slick (OFS) oil prepared as a high energy water-accommodated fraction (HEWAF). Test G001 failed to produce a dose response due to insufficient mortality; therefore, test G002 was performed as a repeat of test G001 using higher nominal HEWAF concentrations. Test G003 was performed with source (MASS) oil prepared as a HEWAF. Transcriptional response data (i.e. RNA-Seq and qPCR) are provided in a separate dataset (UDI: R4.x258.258:0001).本研究针对新鲜受精的鲯鳅（mahi-mahi）胚胎，采用两种原油（浮油（OFS）与原油源油（Mass））开展了3组96小时时间序列暴露实验，实验目的如下： 1. 通过RNA测序（RNA-Seq）与实时荧光定量PCR（qPCR）分析全生物体转录组应答特征； 2. 通过对心率、心包水肿及卵黄囊水肿进行成像分析，实现转录组应答与心脏毒性终点的表型锚定； 3. 获取额外的qPCR重复样本，以验证经RNA-Seq筛选得到的目标基因的转录组应答情况。 本数据集涵盖本次暴露实验及表型分析的全部数据，同时包含两种不同风化状态原油的毒性阈值（如25%致死浓度（LC25）、50%致死浓度（LC50））。 实验G001与G002均采用经制备为高能量水容馏分（HEWAF）的浮油OFS开展。因死亡率不足，实验G001未获得有效剂量反应关系，故采用更高标称HEWAF浓度重复开展实验G002。实验G003采用经制备为HEWAF的源油Mass开展。 转录组应答数据（即RNA-Seq与qPCR数据）已收录于独立数据集，其唯一数据集标识符（UDI）为R4.x258.258:0001。