Whole lung transcriptome from lung epithelial IRE1a knockout mice

This experiment sought to characterize the whole lung transcriptional changes induced by loss of IRE1a function in the lung epithelium. Mice were Shh(Cre/+) IRE1a(fl/fl) on the C57BL/6 background, and had loss of IRE1a function in the epithelium based on XBP1 splicing activity. Lungs from uninjured mice were harvested, flash-frozen, homogenized, and RNA isolated by Trizol extraction. Overall design: Principal treatment groups are: IRE1a epithelial knockout (Shh Cre/+ IRE1a fl/fl, 5 mice) and IRE1a flox (Shh +/+ IRE1a fl/fl, 5 mice).