NOX1 is essential for TNFa-induced intestinal epithelial ROS secretion and inhibits M cell differentiation [scRNA-seq]

Loss-of-function mutations of NOX1 is associated with inflammatory bowel disease (IBD). Mechanisms whereby loss of Nox1 drives IBD are incompletely defined. Here we used TNF? treated colonoids to model IBD inflammation followed by single cell sequencing to get transcriptome profile of IBD relevant cells. Overall design: Single cell seq analysis of mice intestinal colonoid from untreated and TNF?-treated samples