Disruption of RNAPII transcription elongation links Oncogenic splicing factor mutations to replciation stress and targetable alterations in chromatin landscape [nascent RNAseq]

As a complementary approach to GROseq, we peformed short read sequencing of nascent RNA (nSRS-seq) in K562 cells (wild type and SF3B1K700E mutant) at 4 days after mutant allele expression We then performed nascent gene transcription expression using data obtained from nSRS-seq Nascent/Chromatin bound RNA differential gene expression in K562 (wild type vs SF3B1K700E mutant) cells