Supplementary material for: "Seeds adapted to mixed cropping increase yield and drought resistance of cereal-legume mixtures"

Supplementary material for the research article: "Seeds adapted to mixed cropping increase yield and drought resistance of cereal-legume mixtures".

Raw data

R analysis code

Statistical analysis info: ANOVA and Tukey comparisons tables

Abstract:

Cropland diversification through mixed cropping has the potential of achieving a more sustainable agriculture while securing food production. This is of special relevance with climate change and the expected drier growing conditions in the future. Seed adaptation to this cropping method is hypothesised to be a fundamental factor to maximise these benefits, as well as the particular species combined. In this study we compared the performance of four cereal-legume mixed crops (wheat and oat mixed with lupin and lentil in pairs) with their respective monocrops. Each crop was sown using seeds adapted to monoculture and mixed cropping, respectively. Moreover, they were grown under early-season and late-season drought treatments and under control conditions. We measured above-ground vegetative biomass, seed yield and harvest index to evaluate crop production, drought resistance and the effect of seed adaptation on each mixed and monocrop. Our results show that mixed cropping either had a beneficial or neutral effect on crop yield, depending on the species combination and drought conditions, but harvest index was generally higher in monocrops. We also confirmed that seed adaptation to a particular type of cropping is clearly a determining factor in its performance. In accordance with the insurance hypothesis, mixed cropping has the effect of protecting crop yields in the case of a sudden bad performance of one of the species, for example, caused by adverse environmental conditions. It is necessary to focus on effective species combinations which have the best responses to mixed cropping. We show for the first time that wheat-lentil mixtures performed poorly, while wheat-lupin showed the most promising results improving yield and drought resistance. Oat mixed crops did not show differences with the respective monocrops, so they can be a viable cropping option as well and benefit from advantages of crop diversity not measured in this study.