Expression analyses of MUG-Chor1 candidate genes in U-CH1 cells.
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RT-qPCR was done on AB7900 TaqMan (Applied Biosystems; Foster City, CA). Normalization (GAPDH and ACTB) and statistical analysis was done with GenEx Professional (MultiD Analysis; Version 5.3.5.6; see also 2.7). Cut-off for multiple testing (ALG11, UCHL3, TMEM144 and PPP2CB) was p = 0.01274.acalculated as mean values from quadruplicate or triplicate (in case the Cq value could not be defined) biological samples.bCq values were normalized to GAPDH and ACTB (ΔCq). Differential expression (ΔΔCq) is given as positive (up-regulated in large cells) or negative (down-regulated in large cells) fold change ( = 2ΔΔCq).
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2015-12-02
