Expression data from monocyte-derived macrophages co-cultured in the presence of multiple myeloma (MM) cell line and/or MM-bone marrow stromal cell (BMSC)

Bone marrow (BM) microenvironment of multiple myeloma (MM) is populated by tumor associated monocytes and macrophages (TAMs), where they protect clonal plasma cells (PC). It is well known that MM cells can modulate the transcriptome of macrophages, thus, we sought to investigate if a key component of the tumor microenvironment such as MM-BMSCs have the potential to modulate the transcriptome of macrophages as MM cells do. We used microarrays to analyze the potential of MM-BMSC to polarize the transcriptome of TAMs in the presence and absence of MM cells MACS sorted CD14+ monocytes from healthy donors were cultured in 12 well plates in the presence of 10 ng/ml of M-CSF for 7 days to differentiate into macrophages (MDM). On the seventh day cells were harvested and co-cultured in the presence of MM cells, MM-BMSC or both for 24 hrs. Afterwards macrophages were FACSsorted and stored in RLT Plus buffer till RNA extraction.