Translatome analysis of brain endothelial cells in the acute epileptic mouse model

In this study, we provide the translatome data in the brain endothelial cells (BECs) of the adult cortex in response to acute neuronal activation by using translating ribosome affinity purification (TRAP) combined with high-throughput RNA sequencing analysis in Tie2-Cre:Ribo-Tag mice, which are designed for expressing hemagglutinin A (HA)-tagged RPL22, a ribosomal protein in endothelial cells depending on CRE recombinase. To increase neural activity rapidly, we adopted an acute epilepsy induction model in which pentylenetetrazol (PTZ), a GABAA receptor antagonist was injected and observed significant upregulation of c-fos-positive cells in the cortex. To obtain a high-quality BEC pool, we modified the TRAP method by adding the capillary isolation process before the immunoprecipitation step and finally verified BEC-specific translating mRNA purification using RT-PCR. We then analyzed RNA-seq data obtained from the BECs of cortex of adult mice injected with phosphate buffered saline (PBS) or PTZ and identified brain endothelial cell genes that are changed dynamically due to neuronal activation. This information will be useful for researchers to study neurovascular interaction or to identify biomarkers in epilepsy. RNA profiles of brain endothelial cells obtained from cortex of adult mice injected with PBS or PTZ.