Deep sequencing of the edited genomic sites after targeted knock-in (exogenous EGFP integrated into the genome)

This study was performed to compare the frequencies of indel formation at the edited genomic sites (on-target sites) upon targeted knock-in using CRISPR/Cas9 nickases and those elicited by the use of CRISPR/Cas9 nucleases. A NLS-ZF-EGFP fusion gene integrated into the genome was subjected to editing and analyzed by deep sequencing.