Liquid chromatographic methods in the determination of inosine monophosphate dehydrogenase enzyme activity: a review: Supplementary files

Inosine monophosphate dehydrogenase (IMPDH) is a crucial enzyme involved in the de novo synthesis
of purine nucleotides. IMPDH activity is used to evaluate the pharmacodynamics/pharmacokinetics of
immunosuppressant drugs such as mycophenolic acid and thiopurines. These drugs are often used to
prevent organ transplant rejection and as steroid-sparing agents in autoinflammatory diseases such
as inflammatory bowel disease and rheumatoid arthritis. Numerous analytical techniques have been
employed to evaluate IMPDH activity in biological matrices. However, hyphenated LC techniques were
most widely used in the literature. This review focuses on hyphenated LC methods used to measure
IMPDH activity and provides detailed insight into the sample preparation techniques, chromatographic
conditions, enzymatic assay conditions, detectors and normalization factors employed in those methods.