APEX-seq of time course of P-body and Stress Granule transcriptomes during stress

The regulation of cytosolic mRNAs during and after stress occurs through the formation of distinct membraneless organelles. However, the compositional and functional dynamics of these structures throughout the stress response remain poorly understood. Here, we combine APEX2-mediated proximity labelling and RNA sequencing to create a time course of the human transcriptomes associated with P-bodies and stress granules during oxidative stress and subsequent recovery.