In this project, we design a methods to detect novel transcripts integrating ab initio prediction on genomic sequences and filtration with various RNA-seq data. 100 splice sites were selected and RT-P
Alternative splicing can lead to distinct protein isoforms. These can have different functions in specific cells and tissues or in different developmental stages. In this study, we explored whether tr
In eukaryotes, alternative pre-mRNA splicing allows a single gene to encode different protein isoforms that function in many biological processes, and they are used as biomarkers or therapeutic target
A-C) Relative abundance of Gckr transcripts-1,2, X1 and X2 in mouse tissues determined using primer sets targeting either a common sequence (spanning exons 14-15) or spanning the deletion sites of tra
