Dataset for: Female reproductive fluids attracts more and better sperm in zebrafish

Mounting evidence shows that the female reproductive fluid (FRF) can differently affect sperm performance of different males by biasing paternity share among competing males. Here, we tested for the first time the potential of ‘within-ejaculate cryptic female choice’ mediated by the FRF in the zebrafish (Danio rerio). Using a recently developed sperm selection chamber, we separated and collected FRF-selected from non-selected sperm to compare the two subpopulations of sperm in terms of sperm number, viability, DNA integrity and fertilizing ability. We showed that the sperm attracted by FRF are more numerous, more viable and with higher DNA integrity. In addition, FRF-selected sperm fertilized more eggs, but if this is due to fertilization ability per se or numerical advantage is unknown. Our results suggest that the FRF can select sperm with a better phenotype, highlighting the crucial and impactful role that the FRF has in the process of fertilization and post-mating sexual selection d..., , Missing data code in data: NA越来越多的研究证据表明，雌性生殖液（female reproductive fluid, FRF）可通过调控竞争雄性间的父权分配比例，对不同雄性的精子表现产生差异化影响。本研究首次以斑马鱼（Danio rerio）为实验对象，探究了由FRF介导的「射精内隐蔽雌性选择（within-ejaculate cryptic female choice）」的潜在效应。本研究借助新近开发的精子筛选腔，将经FRF筛选的精子与未筛选精子分离并收集，随后从精子数量、活力、DNA完整性及受精能力四个维度对这两类精子亚群展开比较分析。研究结果显示，被FRF吸引的精子数量更多、活力更强且DNA完整性更高。此外，经FRF筛选的精子可使更多卵完成受精，但目前尚不清楚这一效果是源于精子自身的受精能力，还是得益于其数量上的优势。本研究结果提示，FRF可筛选出表型更优良的精子，凸显了其在受精过程及交配后性选择中发挥的关键且重要的作用……数据集中的缺失值编码为NA。