Cortical bulk RNA sequencing of the microglia specific TREM2 inducible mouse models

We performed brain bulk RNA-seq aiming to study how microglia TREM2-WT or TREM2-R47H expression affect the overall brain transcriptomic profiles. Using WGCNA gene network analysis, we found the presynaptic transmission pathway was dysregulated after TREM2-R47H expression, not TREM2-WT. This finding was consistent with the impaired presynaptic transmission detected by electrophysiological recording in TREM2-R47H expressing mice. In addition, dysregulated circadian pathways were also identified in TREM2-R47H mice, not in TREM2-WT mice. Altogether, our bulk RNA-seq results provide additional evidence at transcriptomics level that TREM2-R47H expression in microglia affects neuronal functions and brain networks. Overall design: Six mice (3 males and 3 females) were analyzed for each of the four groups, including TREM2-WT-Ctrl, TREM2-WT-Induced, TREM2-R47H-Ctrl, TREM2-R47H-Induced.