Microbial enzyme activities: peptidase activities in bulk seawater samples from the RV\Polarstern cruise ARKXXVII/3 in the Central Arctic Ocean and Laptev Sea, Aug-Sept. 2012

<p>This dataset includes peptidase activities measured in bulk (not filter-fractionated) seawater. Links to archived CTD data are also provided.&nbsp;Five substrates linked to a 7-amido-4-methyl coumarin (MCA) fluorophore, one amino acid – leucine – and four oligopeptides – the chymotrypsin substrates alanine-alanine-phenylalanine (AAF) and alanine-alanine-proline-phenylalanine (AAPF), and the trypsin substrates glutamine-alanine-arginine (QAR) and glutamic acid-gylcine-arginine (EGR) – were used to measure exo- and endo-acting peptidase activities, respectively.</p>本数据集收录了未经滤组分分级的总体海水中测得的肽酶活性，同时提供了存档CTD（Conductivity, Temperature, Depth）数据的链接。本次实验共使用五种与7-酰胺基-4-甲基香豆素（7-amido-4-methyl coumarin, MCA）荧光基团相连的底物，其中包括1种氨基酸——亮氨酸，以及4种寡肽：分别为胰凝乳蛋白酶底物丙氨酸-丙氨酸-苯丙氨酸（AAF）、丙氨酸-丙氨酸-脯氨酸-苯丙氨酸（AAPF），以及胰蛋白酶底物谷氨酰胺-丙氨酸-精氨酸（QAR）和谷氨酸-甘氨酸-精氨酸（EGR），上述底物分别用于测定外切肽酶与内切肽酶的活性。