20240820_TEM
收藏Figshare2024-12-20 更新2026-04-08 收录
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https://figshare.com/articles/dataset/20240820_TEM/28072376/1
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资源简介：
Transmission electron microscopy of cells were grown overnight and then sub-cultured into fresh LB, grown at 37°C shaking for 3 hours before being rinsed and then resuspended in TEM buffer and delivered for preparation.Wild-type MG1655 (MJG0001) and <i>ppk relA spoT </i>(MJG1282) were grown in LB at 37°C until exponential phase growth (approximately OD600 0.3–0.5). Cells were then spun down and collected. Remove media from pellet and fixin 1% Osmium tetroxide (148) in 0.1M Sodium Cacodylate BufferpH 7.4 at room temperature in the dark for 1 hour, then 3 times 0.1M Sodium Cacodylate BufferpH 7.4 rinse for 15 minutes each. 1% Low molecular weight tannic acid (Ted Pella Inc) for 20 minutes, 3 times 0.1M Sodium Cacodylate BufferpH 7.4 rinse for 15 minutes each.
提供机构：
Hamm, Christopher; Gray, Michael
创建时间：
2024-12-20
