Sixty-seven isolates of global origins used in this study.

aStrain ID in the Francisella Strain Collection (FSC) and Francisella DNA Collection (FDC), Swedish Defense Research Agency, Umeå, Sweden. bStrain ID in the Northern Arizona University DNA collection. cMLVA-defined groups presented in Johansson et al. 2004. A1, F. tularensis subsp. tularensis subpopulation A1; A2, F. tularensis subsp. tularensis subpopulation A2; B, F. tularensis subsp. holarctica; M, F. tularensis subsp. mediasiatica; N, F. novicida. dThe isolates FDC079, FSC119, FSC184, FSC663, and FSC775 (F. philomiragia subsp. noatunensis) were typed with the final one-plate assay and were not part of the set of 62 isolates used in the developing stage. eThe isolates FSC017 (B5), FSC033 (A1), FSC147 (M), FSC156 (N), FSC257 (B3), and FSC641 (B2) were typed with the final one-plate assay, and were part of the set of 62 isolates used in the developing stage, to confirm the typing accuracy of the plate. fFSC257 is an alternative name for RC503.