Detoxification of aflatoxin B1 by essential oils of medicinal herbs

The essential oils (EO) extracted from the medicinal plants viz., Heliotropium bacciferum, Ocimum dhofarense and Zataria multiflora were diluted with methanol (1:10, v/v) and 250 μl of EO was mixed with AFB1 (50 μg/l) in a microcentrifuge. Following a 24-h incubation at 25°C, AFB1 in the mixture was extracted with 250 μl of chloroform. The chloroform fraction was evaporated at 60°C using a water bath and the residue was quantified by enzyme-linked immunosorbent assay using a commercial kit (RIDASCREEN Aflatoxin B1; R-Biopharm AG, Darmstadt, Germany).从药用植物碟花天芥菜（Heliotropium bacciferum）、佐法尔罗勒（Ocimum dhofarense）及多花百里香（Zataria multiflora）中提取的精油（essential oils, EO）以甲醇按体积比1:10（v/v）进行稀释，随后取250微升该精油溶液与50微克/升的黄曲霉毒素B1（AFB1）在微量离心管中混合。于25℃条件下孵育24小时后，使用250微升三氯甲烷对混合体系中的AFB1进行萃取。将三氯甲烷萃取相置于60℃水浴中蒸干，随后采用商用试剂盒（RIDASCREEN Aflatoxin B1；R-Biopharm AG，德国达姆施塔特）通过酶联免疫吸附试验（enzyme-linked immunosorbent assay）对所得残留物进行定量检测。