AtT-20 cell line expression data

Results of transcript sequencing for AtT-20FlpIn cells. mRNA was isolated from AtT-20FlpIn cells using standard procedures, next generation sequencing was performed by Macrogen (https://dna.macrogen.com/). A report ourtlining the workflow and data analysis methods is available from the Authors by request. Deposited data is in an Excel file, which includes the gene symbol, transcript ID from the reference mouse genome, protein ID and transcript abundance. The AtT-20FlpIn cells were generated by Dr Santiago, and have been used as the 'wild type' cells for generating cell lines stably expressing GPCR and ion channels for most of the molecular pharmacology projects in the Molecular Pharmacodynamics group.AtT-20FlpIn细胞的转录组测序结果。研究人员采用标准实验流程从AtT-20FlpIn细胞中分离mRNA，并由Macrogen公司（https://dna.macrogen.com/）完成下一代测序。有关实验流程与数据分析方法的详细报告可应要求向作者索取。已上传的数据集存储于Excel文件中，内容涵盖基因符号、参考小鼠基因组对应的转录本ID、蛋白质ID以及转录本丰度信息。AtT-20FlpIn细胞由圣地亚哥博士构建，在分子药效动力学研究组的多数分子药理学相关项目中，该细胞均作为“野生型”亲本细胞，用于构建稳定表达G蛋白偶联受体（GPCR）与离子通道的细胞系。