Retinoic acid signaling modulation guides in vitro specification of human heart field-specific progenitor pools

He we report a systematic analysis of RA impact on human cardiovascular progenitor specification in orchestry with other signaling cues during early mesoderm formation. To approach this, we used a single cell transciptome profiling to dissect heterogeneity within human heart field-like cell populations and their derivatives. Our analysis enable us to identify known as well as a novel population of cells that can in vitro differentaite towards myocytic as well as epicardial lineages. single cell transcriptome analysis of hESC in vitro derived human primitive streak-like cells (d1.5), cardiovascular progenitors (4 sorted samples at d4.5) and their derivatives (4 samples at d30)