RNA-seq of etv2 mutant knock-in heterozygous and homozygous zebrafish embryos

Zebrafish etv2^Gt(2A-Gal4)ci32 line was generated using CRISPR-Cas9 mediated NHEJ approach which has 2A-Gal4 sequence inserted with the exon 5 of etv2 gene thus interrupting etv2 coding sequence. Heterozygous and homozogous embryos were subjected to bulk RNA-seq 3 batches of 15-20 heterozygous and homozygous embryos were frozen at the 15-somite and 24 hpf stages. Total 12 samples were sequenced: triplicate samples of Heterozygous and homozygous embryos at 15-somite and 24 hpf stages