Expanded gene and taxon sampling of diplomonads shows multiple switches to parasitic and free-living lifestyle.

Expanding the taxon sampling of phylogenomic analyses for diplomonads by sequencing transcriptomes of thirteen free-living isolates and one endobiotic isolate.Constructing a well-resolved evolutionary framework for exploring lifestyle adaptation in diplomonads using a phylogenomic approach.Identifying genetic signatures of parasitic ancestry (variant-specific surface proteins, virulency factors) in free-living diplomonads.