Gene expression profiling in response to strigolactone signalling revealed by dexamethasone treatment to SL-insensitive version of SMXL7 tagged with glucocorticoid receptor (SMXL7d53-GR) in Arabidopsis hypocotyl.

Strigolactone (SL) signalling plays many roles in plants, however, the downstream responsive genes remain unclear. SUPPRESSOR OF MORE AXILLARY GROWTH2-LIKE 7 (SMXL7) is degraded upon SL signalling. Here, we generated Arabidopsis carrying a transgene SMXL7pro:SMXL7d53-GR, where a SL-insensitive version of SMXL7 is tagged with the ligand binding domain of the glucocorticoid receptor (GR). SMXL7d53-GR will be translocated to the nucleus upon dexamethasone (dex), and we profiled the gene expression in mock or dex treated plants and revealed the downstream responsive genes of SL signalling. Overall design: RNA-seq analysis of mock or dex treated SMXL7pro:SMXL7d53-GR transgenic Arabidopsis. Four replicates for each mock or dex treated sample.