Genetic variants and cell-specific signatures in PBMCs of pre transplant recipients

Long-term survival of the renal allograft is a challenge. A single cell sequencing analysis is a powerful approach to identify genetic/genomic risk for development of long-term graft loss at single cell level. In this study, we compared single cell RNA sequencing profiles of pre-transplant PBMC samples from 4 transplant patients (2 patients with graft loss within 3 years vs 2 patients without graft loss) with simultaneous genome-wide genotype data for common variants to idenitfy the immune cell subtypes and transcriptomic signatures associated with long-term graft loss. These transcriptomic data also integrates with genetic data in an effort to identify genes that mediate the risk for graft survival in a cell-type specific context. 4 PBMC samples: two with graft loss within 3 years and the other two without graft loss