BioPlex 2.0 ( ~ 56,000 interactions )

The BioPlex (biophysical interactions of ORFeome-based complexes) network is the result of creating thousands of cell lines with each expressing a tagged version of a protein from the ORFeome collection. Immunopurification of the tagged protein and detection of associated proteins by mass spectrometry are the building blocks of the network. The overarching project goal is to determine protein interactions for every member of the collection. Our current release has more than 5,000 human proteins as baits and covers ~56,000 interactions. Edge color intensity is mapped to the p(Int) value for each interaction.