BHCL_DMPC_Na

This dataset contains NMR spectra obtained for the sample -BHCL_DMPC_Na Nucleus: 13C NMR Solvent: H2O+D2O NMR Probe: H8609_0043 (PH MAS 500WB BL4 X/Y/H DVT) NMR Pulse Sequence: null Temperature: 295.9874 Observed Frequency: 125.78746 Magnetic Field Strength: 11.747332789100614 Number of Scans: 2048 NMR Pulse Sequence: C90.hpdec Spectral Width: 189.267675585541 Number of Data Points: 2378 Relaxation Delay: 6 Observed Frequency: 125.7981519341 Nucleus: 13C NMR Solvent: H2O+D2O NMR Probe: H8609_0043 (PH MAS 500WB BL4 X/Y/H DVT) NMR Pulse Sequence: null Temperature: 295.9961 Observed Frequency: 125.78746 Magnetic Field Strength: 11.747332789100614 Number of Scans: 2048 NMR Pulse Sequence: NEA_ineptrd Spectral Width: 180.666405933068 Number of Data Points: 2270 Relaxation Delay: 6 Observed Frequency: 125.7968940595 Nucleus: 1H NMR Solvent: H2O+D2O NMR Probe: H8609_0043 (PH MAS 500WB BL4 X/Y/H DVT) NMR Pulse Sequence: null Temperature: 295.9987 Observed Frequency: 500.248 Magnetic Field Strength: 11.749122218330188 Number of Scans: 2 NMR Pulse Sequence: onepulse Spectral Width: 19.9900065723262 Number of Data Points: 3006 Relaxation Delay: 5 Observed Frequency: 500.24996059 Nucleus: 31P NMR Solvent: H2O+D2O NMR Probe: H8609_0043 (PH MAS 500WB BL4 X/Y/H DVT) NMR Pulse Sequence: 1d Temperature: 295.9902 Observed Frequency: 202.504102 Magnetic Field Strength: 11.745385617451017 Number of Scans: 256 NMR Pulse Sequence: NEA_zg30bbdec Spectral Width: 201.284167395372 Number of Data Points: 4886 Relaxation Delay: 6 Observed Frequency: 202.504102 Nucleus: 31P NMR Solvent: H2O+D2O NMR Probe: H8609_0043 (PH MAS 500WB BL4 X/Y/H DVT) NMR Pulse Sequence: 1d Temperature: 295.9978 Observed Frequency: 202.504102 Magnetic Field Strength: 11.745385617451017 Number of Scans: 2048 NMR Pulse Sequence: NEA_zg30bbdec Spectral Width: 201.284167395372 Number of Data Points: 4072 Relaxation Delay: 6 Observed Frequency: 202.504102 Nucleus: 13C,1H NMR Solvent: H2O+D2O NMR Probe: H8609_0043 (PH MAS 500WB BL4 X/Y/H DVT) NMR Pulse Sequence: null Temperature: 295.9881 Observed Frequency: 125.78746,500.248 Magnetic Field Strength: 11.747332789100614 Number of Scans: 256 NMR Pulse Sequence: NEA_RPDLF Spectral Width: 180.666405933068,4.99752988789391 Number of Data Points: 2270,32 Relaxation Delay: 6 Observed Frequency: 125.7968940595,500.2471333

BHCL (10.2mg, 1.0 eq.) was added to an eppendorf vial and dissolved with D54-DMPC (21.6mg,
4.0eq.) in 1.98ml chloroform and 0.85ml methanol. The solvent was evaporated at 40°C under
a constant stream of argon and the predried sample was freeze-dried over night. 23.15µl degassed
water was added and the Eppendorf was stored at 50°C for one day. Lost water was added and
the sample was mixed with ten freeze-thaw cycles (1min liquid N2 and 3min in 50°C ultra sound
bath). After another day of equilibration the sample was packed in the insert.