A Novel Grape Downy Mildew Resistance Locus from Vitis rupestris

This study represents the QTL mapping efforts for downy mildew resistance in grapevine using a wild V. rupestris x V. riparia F1 mapping family. Genotyping by sequencing was used to generate genome wide SNP markers based on the APEK1 restriction sites in the grape genome. Curation of raw data through alignment to the published grape genome, and subsequent quality filtering, resulted in a core set of markers in this family. A genetic map was produced and the phenotype of downy mildew resistance was assessed for QTL.

本研究旨在利用野生品种 V. rupestris 与 V. riparia 的 F1 杂交后代，对葡萄抗霜霉病性状的 QTL 进行定位分析。通过测序进行基因分型，基于葡萄基因组中的 APEK1 限制性酶切位点，生成全基因组范围的 SNP 标记。通过对原始数据进行与已发表葡萄基因组的比对以及后续的质量筛选，得到该家族的核心标记集。构建了遗传图谱，并对霜霉病抗性性状进行了表型评估以确定 QTL。