Additional file 1 of High temperatures and low humidity promote the occurrence of microsporidians (Microsporidia) in mosquitoes (Culicidae)

Additional file 1: Figure S1: Correlation plots between the environmental variables: (A) temperature vs. humidity, (B) temperature vs. wind (C), temperature vs. rainfall (D) humidity vs. wind, (E) humidity vs. rainfall, (F) wind vs. rainfall. Figure S2: Phylogenetic tree of Microsporidia inferred from BI and ML analyses of concatenated ssu rRNA gene sequence data. Values near branches show Bayesian posterior probabilities (PP) and bootstrap support values (BS) (PP/BS). Black circles: maximally supported; empty circles: supported > 0.95 PP and > 75% BS. Sequences found in this study are in bold. Figure according to Trzebny et al. (2023): 10.1016/j.jip.2022.107873. Figure S3: Correlation plots between microsporidian-positive mosquitoes and (A) temperature (°C), (B) humidity (%), (C) wind (m/s), and (D) rainfall (mm). The R value indicates the Pearson’s correlation coefficient statistic, the p value is statistically significant, the shadowed area shows the 95% confidence interval, and the black line is the regression line. The month and year of the data points are indicated according to the legend at the bottom. Figure S4. Host mosquito species in which particular microsporidian species were found. Vertical axis: percentage of a given mosquito host.Table S1: Daily values of temperature, humidity, wind and precipitation registered in the study area. Table S2: Descriptive values (mean, standard deviation, minimum and maximum) of each environmental factor. Table S3: Number of mosquitoes collected in each month of the study. Table S4: GenBank accession numbers of the COI sequences found in this study. Table S5: Percentage of mosquitoes collected in each month of the study. Table S6: Correlation values between climatic variables and the occurrence of mosquitoes. Table S7: GenBank accession numbers of the V5 18S rRNA gene sequences found in this study. Table S8: Number of mosquitoes positive for microsporidian DNA. Table S9: Percentage of mosquitoes positive for microsporidian DNA. Table S10: Number of mosquitoes divided by sex, positive for microsporidian DNA. Table S11: Percentage of mosquitoes divided by sex positive for microsporidian DNA. Table S12: Two-way ANOVA F distribution for the relationships between microsporidians detected in mosquitoes and sex of mosquitoes, month (cold-warm) and both factors. Table S13: Indicator species analysis for season. Microsporidians that are significant are shown. The “A component” indicates how exclusive the species was for season, where a value of 1 indicates that the species was exclusively found in that group. The “B component” indicates how frequently the given species was found in replicate samples, where 1 means it was found in every sample. Table S14: Spearman correlation coefficients (rho) for the relationships between microsporidians detected in mosquitoes and environmental factors. Table S15: The number of observed occurrence and co-occurrence in the analysed mosquitoes. Table S16: Identified co-occurring microsporidia in each individual. Table S17: Frequency of co-occurrence pairs in females and males divided by season. The pairs noted in both warm and cold seasons are shown.