Flongle data for KnowYourCG: Facilitating base-level sparse methylome interpretation

ONT-based 5mC and 5hmC signals across four mouse tissues (lung, blood, uterus, and cortex) profiled with low-pass Flongle flowcells (~1M CpGs per sample). Decoding DNA methylomes for biological insights is critical in epigenetics research. We present KnowYourCG (KYCG), a data interpretation framework designed for functional DNA methylation analysis. Unlike existing tools that target genes or genomic intervals, KYCG features direct base-level screenings of diverse biological and technical influences, including sequence motifs, transcription factor binding, histone modifications, replication timing, cell-type-specific methylation, and trait associations. Through implementing efficient infrastructure that rapidly screens and investigates thousands of knowledge bases, KYCG addresses the challenges of data sparsity in various methylation datasets, including low-pass or single-cell DNA methylomes, 5-hydroxymethylation profiles, spatial DNA methylation maps, and array-based datasets ..., , # Flongle data for KnowYourCG: Facilitating base-level sparse methylome interpretation

Dataset DOI: [10.5061/dryad.zgmsbccq9](10.5061/dryad.zgmsbccq9)

## Description of the data and file structure

Oxford Nanopore Technology (ONT) is an emerging approach to directly discriminate 5mC, 5hmC, and unmodified C from ion current signals, bypassing cytosine deamination methods that cannot separate 5mC and 5hmC. Here, we provide ONT-based 5mC signals across four mouse tissues (lung, blood, uterus, and cortex) profiled with low-pass Flongle flowcells (~1M CpGs per sample). Each of the four mouse tissues is stored as .cg file to capture the methylation information. 

### Files and variables

#### File: uterus.tar

**Description:** uterus 20231207_1654_MN36407_AQY317_deaa9eb5

* Contents: One `.cg` file (`uterus_20231207_1654_MN36407_AQY317_deaa9eb5.cg`)
* Tissue type: Mouse uterus
* Sequencing date: 2023-12-07
* Flowcell ID: MN36407
* Experiment tag: AQY317
* Approx. coverage: ~1M CpGs
* Notes:...,