Gene expression profiling of fetal liver-derived hematopoietic stem cells in conventional ESAM-knockout mice.

Endothelial cell-selective adhesion molecule (ESAM) is a life-long maker for hematopoietic stem cells (HSCs). Although we previously elucidated the functional importance of ESAM on HSCs in stress-induced hematopoiesis in adults, it remained unknown how ESAM affects hematopoietic development during fetal life. To address this issue, we analyzed fetuses of conventional ESAM-knockout mice. Overall design: We conducted RNA-Seq comparing gene expression patterns of WT and ESAM-null HSCs to learn more about molecular mechanisms involved in the developmental failure of hematopoiesis in the ESAM-null fetal liver.