Supplemetary data for the paper "Knockdown of circular RNA hsa_circ_0003307 inhibits synovial inflammation in ankylosing spondylitis by regulating the PI3K/AKT pathway" by Yanyan Fang, Jian Liu*, Yan Long, Jianting Wen, Dan Huang, Ling Xin

Statistical analyses and graphs were performed using Prism 8 software (GraphPad, La Jolla, USA). Age was analyzed using Student's t test (Supplementary Table 1A,B). Expression of circRNA_0003307 in PBMCs was analyzed using Welch's t-test (Supplementary Table 2A,B). CircRNA_0003307 expression in AS-FLS was analyzed using a one-way analysis of variance, then Games-Howell test (Supplementary Table 4A–C). The p-PI3K protein expression in AS-FLS was analyzed using a one-way analysis of variance, then Games-Howell test (Supplementary Table 5A–C). The p-AKT protein expression in AS-FLS was analyzed using a one-way analysis of variance, then Games-Howell test (Supplementary Table 6A–C). The TNF-α levels in AS-FLS was analyzed using a one-way analysis of variance, then Games-Howell test (Supplementary Table 7A–C). The TNFAIP2 levels in AS-FLS was analyzed using a one-way analysis of variance, then Games-Howell test (Supplementary Table 8A–C). Categorical variables were compared using chi-square test. Correlations were assessed using Spearman’s analysis because clinical characteristics were not normally distributed (Supplementary Table 3). A receiver operating characteristic (ROC) curve analysis was performed to judge whether circRNA_0003307 can be used as a diagnostic indicator for AS. P <0.05 indicates that the difference was statistically significant.