Concentrations of chlorophyll-alpha combined with different size fractions of microplankton in sea water from the euphotic zone of the Aegean Sea at Station MARCH-1997-GN36199704603MNB01

Samples from 2 l of sea water were size fractionated by filtration through polycarbonate Millipore filters with pores 0.2 µm, 1.2 µm, and 3.0 µm. The filters were kept deep frozen and analyzed according to Holm-Hansen et al. (Holm-Hansen, O., Lorenzen, C.J., Holmes, R.W., Strickland, J.D.H. 1965. Fluorometric determination of chlorophyll. Journal du Conseil Permanent International Pour L'Exploration de la Mer 30, 3-15).采集2升海水样本，通过孔径分别为0.2 μm、1.2 μm与3.0 μm的密理博（Millipore）聚碳酸酯滤膜过滤以完成粒径分级。将滤膜深冻保存，并依照Holm-Hansen等人的方法开展分析（Holm-Hansen, O., Lorenzen, C.J., Holmes, R.W., Strickland, J.D.H. 1965. 叶绿素的荧光测定法. 《国际海洋考察理事会期刊》，30卷：3-15）。