Kyle E. Miller, Michael P. Sheetz (2011) CIL:31203, Gallus gallus, neuron. CIL. Dataset

Mitochondrial transport during a growth cone pause. Chicken DRG neurons were grown on coverslips coated with laminin/poly-L-ornithine for 2 d and stained with 0.1 µM MitoTracker Red CMXRos. Images were acquired every 2 s. To reduce the size of the move, one frame for every 8 s is shown. In the movie, the growth cone is toward the right-hand side. The time elapsed is indicated in the top left hand corner. During the first 10 min of the movie, the growth cone advances. It then pauses, but low velocity anterograde transport of the docked mitochondria continues unabated. This movie illustrates low velocity transport continues during prolonged growth cone pauses. Bar, 10 µm.

Images were acquired on a confocal system (FV300 FluoView; Olympus) on an IX70 microsocpe using a 60×, 1.4 NA, PlanApo objective. Samples were illuminated with an argon/krypton Omnichrome 488/568/647 line laser set to standby mode, with the laser intensity set at 6%, the confocal aperture set at 5, and pixel dwell times of either 100 or 200 microsec; the light was further attenuated with neutral density filters (either 25 or 50%). Cells were observed in an enclosed flow chamber. Temperature was maintained at 37°C. Movie corresponds to Video 3 in J Cell Biol. 2006 May 8;173(3):373-81.

线粒体在生长锥暂停期间的运输过程。鸡DRG神经元在涂有层粘连蛋白/多L-鸟氨酸的载玻片上培养2天后，用0.1 µM的MitoTracker Red CMXRos进行染色。图像每隔2秒采集一次。为减少移动画面的大小，每8秒展示一帧。在视频中，生长锥位于右侧。时间流逝由右上角标注。在视频的前10分钟内，生长锥向前推进。随后，生长锥暂停，但停靠的线粒体的低速度向前运输持续进行。此视频展示了在长时间的生长锥暂停期间，低速度运输持续进行的情况。标尺，10 µm。图像是在IX70显微镜上使用60×，1.4 NA，PlanApo物镜的共聚焦系统（FV300 FluoView；Olympus）下采集的。样品被设置为待机模式的氩/氪Omnichrome 488/568/647线激光照亮，激光强度设定为6%，共聚焦孔径设定为5，像素驻留时间为100或200微秒；光线通过中性密度滤光片（25或50%）进一步衰减。细胞在封闭的流动室中观察。温度维持在37°C。该视频对应于J Cell Biol. 2006年5月8日第173卷第3期的Video 3：373-381。