Transcriptome differences between immortalized brown preadipocytes treated with different paracrine FGFs

We analyzed coding transcript abundance in primary, immortalized brown preadipocytes from murine, interscapular brown adipose tissue. Overall design: We prepared primary preadipocytes from the stromal-vascular fraction of murine interscapular brown fat of 129SvEv mice and immortalized these by the SV40 large T antigen method. Cells were treated with 5.56nM Fgf1, Fgf8b or FGF9 for 48h in the presence of 1µg/ml heparin. One group of Ffg8b treated cells was also treated with 2µM Bay-876. We sequenced total RNA and determined transcript abundance.