Regulation of TLR8-activated gene expression in human neutrophils [ChIP-seq]

The transcription factors that regulate gene expression in stimulated neutrophils are poorly characterized. Herein, we found that the genomic distribution of the myeloid lineage determining transcription factors PU.1 and C/EBPβ, as well as that of the histone modification H3K27Ac, which marks active chromatin, significantly change in human neutrophils treated with R848, a ligand of TLR8. Examination of histone modification, transcription factor binding and transcriptome in human neutrophils and granulocytic-differentiated HL-60 cell line