Comparison of the single-gene deletions that were identified in this study to result in specific amino acid auxotrophies of E. coli with published information on predicted and empirically tested amino acid auxotrophies.

1 = Tepper & Shlomi (2011), 2 = Baba (2006), 3 = Li & Ricke (2003), ‘ = strain shows weak growth.The effect of the corresponding deletion is indicated as: A strongly reduced growth of mutant on minimal medium, B mutant does not grow on minimal medium, C mutant cannot transform any other amino acid into the focal one, and ns degree of auxotrophy not specified.