Maternal Schistosomiasis CD45+

Maternal helminth infections are a global public health concern and correlate with altered infant immune responses to some childhood immunizations, but a mechanistic understanding of how maternal helminth infection alters the cellular immune responses of offspring is lacking. Here we establish a model of maternal Schistosoma mansoni infection in dual IL-4 reporter mice. We find that offspring born to mothers infected with S. mansoni have impaired production of IL-4 during homoeostasis, and following immunization with a Tetanus-Diphtheria vaccine. Single-cell RNASeq analysis of immunized offspring identified egg antigen-dependent reductions in B-cell cell cycle and proliferation-related genes. Overall design: Offspring from S. mansoni patently infected, S. mansoni single-sex infected (adults only), and mock infected control mothers were immunized with 1/10th the human dose of a comercial tetanus/diphtheria vaccine. At Day 7 post immunization single-cell suspension form 6-8 draining lymph nodes per group were pooled and stained with Dapi (Sigma) and anti-CD45. Live CD45+ cells were sorted via BD FACSAria cell sorter and washed once in PBS containing 0.04% BSA. Samples were then processed for SCseq via a 10× platform (single cell 3'V2) according to the manufacturer's instructions (10× Genomics). Paired-end RNASeq (125 cycles) was performed via an Agilent HiSeq next- generation sequencer. S