OpcA and PorB are novel bactericidal antigens of the 4CMenB vaccine in mice and humans

In this study, we describe the development and use of an ad hoc protein microarray to study the immune response induced by the OMV component of 4CMenB vaccine in infant sera before and after vaccination A panel of recombinant meningococcal proteins were printed randomly in replicates on a non-commercial protein microarray. With the expection of NadA, GNA2091-fHbp and NHBA-GNA1030 (that are tagless), all the proteins were expressed as FLAG- and His-tagged fusions. Recombinant antigens were expressed in E. coli and then purified either from the cytoplasmic fraction as soluble proteins or resolubilized from the inclusions bodies as insoluble forms. Controls consisted of 8 serial 2-fold dilutions of human IgG, proteins expressed and purified from E. coli following the same expression and purification protocol, but originating from different pathogens other than MenB and PBS + 40% glycerol spots. Sera were profiled for anti-Human IgG specific analysis of reactivity to the spotted proteins.