Correlation of virus and cell morphology during the time course of MARV infection.

Quantification of virus morphology in cell culture supernatants was done by examination of thawed cryosections of pelleted virus in a systematic random manner. Note that filamentous viruses are predominantly released at day 1–2 p.i., while spherical viruses are predominantly released at day 3–4 p.i.. Cell morphology and morphology of cell-associated virus was assessed by examination of plastic-embedded thin sections of MARV-infected cells, embedded in their in situ orientation, in a systematic random sampling manner. Note the decrease in amount of filamentous virus and of intact cell profiles from day one to day four p.i., and the increasing amount of spherical virus and vesiculated cell profiles over the same time course. For further details see Results section.