Single Cell Dataset

ControlControl groups tumor were processed into single-cell suspensions when the tumor size of the control group reached 100 mm2. After antibody staining , tumor CD45.2+ cells were sorted on a FACS AriaIII.Ten thousand cells were mixed with 10× Genomics Chromium single-cell RNA mastermix, followed by loading onto a 10× Chromium chip according to the manufacturer’s protocol to obtain single-cell cDNA. Libraries were subsequently prepared and sequenced using a NovaSeq sequencer (Illumina).<br>MK-801Hepa1-6BL (5 × 105) tumor cells were injected subcutaneously (s.c.) into C57BL/6 WT, respectively.Tumor-bearing mice were treated with MK-801 (0.2 mg/kg, i.p. daily from day 5). When the tumor size of the control group reached 100 mm2. After antibody staining , tumor CD45.2+ cells were sorted on a FACS AriaIII. Ten thousand cells were mixed with 10× Genomics Chromium single-cell RNA mastermix, followed by loading onto a 10× Chromium chip according to the manufacturer’s protocol to obtain single-cell cDNA. Libraries were subsequently prepared and sequenced using a NovaSeq sequencer (Illumina).