Transcriptomic Analysis of Cortical and Medullary Thick Ascending Limb of Henle's loop (CTAL and MTAL) from WT or Cldn-10 Knock-out mice

We applied RNAseq analysis on microdissected cortical and medullary thick ascending limb of Henle's loop from WT and Cldn10 KO mice (cKO). Among 11369 genes sequenced 291 have an expression significantly modified (p<0.05) between MTAL and CTAL from WT mice. In cKO mice, among 12023 genes sequenced 359 were signicantly modified (p<0.05) between MTAL and CTAL. surprisingly, while 637 genes have significantly (p<0.05) different expression between MTAL WT and cKO only 66 were in this case between CTAL WT and cKO. Comparison of gene abundance by RNA-sequencing between CTAL and MTAL from WT or Cldn10 knock out mice.本研究对野生型（Wild Type, WT）及Cldn10基因敲除（Cldn10 knockout, KO）小鼠（本研究中为条件性敲除小鼠，cKO）的显微切割肾皮质亨利袢粗段升支与肾髓质亨利袢粗段升支样本，实施了RNA测序（RNA-sequencing）分析。在野生型小鼠的髓质粗段升支（medullary thick ascending limb, MTAL）与皮质粗段升支（cortical thick ascending limb, CTAL）之间，本次测序覆盖的11369个基因中，共有291个基因的表达水平发生显著改变（p<0.05）。在cKO小鼠中，本次测序覆盖的12023个基因里，有359个基因在MTAL与CTAL之间存在显著表达差异（p<0.05）。值得注意的是，MTAL层面的WT与cKO小鼠间，共有637个基因的表达存在显著差异（p<0.05）；而CTAL层面的WT与cKO小鼠间，仅66个基因出现此类表达差异。本数据集通过RNA测序比较了野生型或Cldn10基因敲除小鼠的CTAL与MTAL之间的基因表达丰度。