eDNA Metabarcoding Data and Conventional Fish and Amphibian Survey Data From the Grand River Watershed

The Grand River watershed is the largest watershed in southern Ontario, and its resident population is expected to increase over 50% by 2050. Very little research to date has focused on using eDNA metabarcoding to monitor fish and amphibian communities in the Grand River watershed. This research will compare results from eDNA metabarcoding against results from conventional fish and amphibian survey methods to develop a field-tested eDNA metabarcoding monitoring protocol specific to the Grand River watershed. This data set is collected for the Pillar 3 project titled Next Generation Solutions to Ensure Healthy Water Resources for Future Generations, under the Global Water Futures Program funded by Canada First Research Excellence Fund. This researcher is co-supervised by Drs. Barbara Katzenback and Paul Craig.格兰德河（Grand River）流域是加拿大安大略省南部规模最大的流域，其常住人口预计至2050年将增长超50%。迄今为止，鲜有研究聚焦于利用eDNA元条形码（eDNA metabarcoding）技术监测格兰德河流域的鱼类与两栖动物群落。本研究将对比eDNA元条形码技术与传统鱼类、两栖动物调查方法的结果，以开发适配该流域的经野外验证的eDNA元条形码监测方案。本数据集由加拿大第一研究卓越基金（Canada First Research Excellence Fund）资助的全球水未来计划（Global Water Futures Program）下属第三支柱项目“保障子孙后代水资源健康的下一代解决方案”（Next Generation Solutions to Ensure Healthy Water Resources for Future Generations）采集。本研究由芭芭拉·卡岑贝克（Barbara Katzenback）博士与保罗·克雷格（Paul Craig）博士共同指导。