yylncT acts as a gatekeeper of the mesodermal transcriptional program by local modulation of DNMT3B [mouse]

Bidirectional transcription is a prevalent feature of eukaryotic promoters. The goal of this study is to identify novel divergent long-coding RNAs during differentiation of human ES to cardiomyocytes. Methods: Paired end RNA-Seq was performed on the tissue obtained from the cardiogenic region of the indicated embroynic stages of mice. Following stages of mouse development were used:- E8,E8.5,E9.5 and E14., Results: Using an optimized data analysis workflow, we mapped about ~70 million sequence reads per sample to the mouse genome (build mm10). Conclusions: Our study represents the detailed analysis of the genome wide identification of divergent long-coding RNA generated by paired end RNA-seq technology. Our results show that NGS offers a comprehensive and more accurate quantitative and qualitative evaluation of long-non coding RNA content within a cell. We conclude that RNA-seq based transcriptome characterization would expedite genetic network analyses and permit the dissection of complex biological functions. We have used 4 different time-point (all in triplicates), covering the key stages of in vivo cardiac development in mice embryos. It includes E8, E8.5, E9.5 and E14.双向转录是真核生物启动子（eukaryotic promoters）的普遍特征。本研究旨在鉴定人类胚胎干细胞（human ES）向心肌细胞（cardiomyocytes）分化过程中新型的双向长链非编码RNA（long-coding RNAs）。 方法：本研究针对小鼠指定胚胎发育阶段的心脏发生区域组织开展双端RNA测序（Paired end RNA-Seq）。 本研究选用的小鼠发育阶段包括：E8、E8.5、E9.5及E14。 结果：通过优化后的数据分析流程（data analysis workflow），本研究将每个样本中约7000万条序列读段（sequence reads）比对至小鼠基因组（mouse genome）版本mm10（build mm10）。 结论：本研究详细分析了通过双端RNA测序技术鉴定得到的全基因组范围内的双向长链非编码RNA。本研究结果显示，下一代测序技术（NGS）可对细胞内的长链非编码RNA含量进行全面且更为精准的定量与定性评估。综上，基于RNA测序的转录组表征可加速遗传网络分析，并助力解析复杂的生物学功能。 本研究共设置4个不同时间节点，每个节点均设置3次生物学重复（triplicates），覆盖小鼠胚胎体内心脏发育的关键阶段，具体包括E8、E8.5、E9.5及E14。