Generation of founder knock-out lines by ends-out targeting.

a.Total estimated number of screening cross progeny screened in each targeting experiment. Because progeny of multiple vials or bottles were pooled and screened together, we did not register the clonality of the preliminary candidates. We assumed that each targeting mutant obtained was due to a distinct targeting event, based on the low HR frequency observed.b.Since all female candidates were discarded in targeting experiments, the adjusted HR frequency should be twice higher than listed here.c.Screening crosses were set up on the normal food first, then transferred to G418 food after two days.d.A dArf6ΔKG#1 deletion allele generated by P-excision was used for complementation assays [7].e.Null allele of P{PZ}Dscam05518 (BL#11412) [13] was used for complementation assays.